摘要
水稻(Oryza sativa L.)幼苗经盐、热激和冷三种不同胁迫预处理均提高了幼苗的抗寒性。与未预处理苗相比,在处理后、低温伤害后和常温下恢复2d的三个时期,不同胁迫预处理苗的可溶性和热不稳定蛋白含量变化趋势甚为相似,但热稳定蛋白含量变化则各有异同。SDS-PAGE图谱分析显示,不同胁迫预处理提高水稻幼苗抗寒性时,其可溶性蛋白、热稳定和热不稳定蛋白组成变化亦各有异同。除诱导出共有的新多肽外,还各自诱导出特有的新多肽。结果表明,植物对不同胁迫的交叉适应存在一定的共同机理,但亦可看出植物对同一种环境胁迫似乎不是以同一的机理去适应。
Changes of proteins in the rice (Oryza saliva L.) seedlings from different stress pre-treatments were compared for exploring the mechanism of cross adaptation to different stresses. The chilling resistances of rice seedlings (under 1℃, 150 μmol·m-2·s-1 PFD for 2 days) were enhanced distinctly by salt, heat shock and cold pretreatments. The survial rates of the pretreated rice seedlings after being recovered from chilling injury for 2 days were greatly higher than those of nonpretreated seedlings. The contents of total soluble protein and heat-unstable protein from the different kinds of pretreated seedlings showed a similar trend of changes, hi comparing with the nonpretreated seedlings, total soluble protein and heat-unstable protein were lower just after pretreatment and chilling injury but became higher after recovery for 2 days. The heat-stable protein in the seedlings pretreated by cold or heat shock was higher than those of the nonpretreated seedlings at all three sampling time (after pretreated; after chilling injury; after recovery for 2 days). However,the change of heat-stable protein in salt pretreated seedlings was similar to the changes of the soluble and heat-unstable proteins. SDS-polyacrylamide gel electrophoresis analysis indicated that the component changes of the soluble, heat-stable and heat-unstable proteins among the three pretreated seedlings had both common newly induced polypeptides and different specific newly polypeptides by comparison with the nonpretreated seedlings. These results suggested that the cross adaptation of plants to different environmental stresses involved certain common molecular mechanism, yet the plants might not adapt the same environmental stress with the same resistance mechanism.
基金
国家自然科学基金
关键词
水稻
幼苗
胁迫
蛋白
抗寒性
Rice seedlings, Different stress, Protein, Cross adpatation, Chilling resistance
