摘要
背景与目的基质金属蛋白酶-9(MMP-9)是一个重要的细胞外基质降解酶,与肿瘤的发生发展生长密切相关。本研究初步探讨MMP-9反义寡核苷酸(MMP-9ASODN)对人肺腺癌细胞A549凋亡及转移能力的影响。方法MTT法检测MMP-9ASODN转染对A549细胞生长的影响;流式细胞术检测MMP-9ASODN对A549细胞周期和凋亡比率的影响;MTT比色法和划痕迁移试验观察MMP-9ASODN对A549细胞体外黏附以及迁移的影响。结果MMP-9ASODN对A549细胞存活率抑制呈浓度和时间依赖性,MMP-9ASODN浓度为600nmol/L、作用48h时抑制作用最明显;与对照组相比,转染MMP-9ASODN的A549细胞凋亡百分比明显升高(P<0.01),而黏附力及迁移细胞数显著降低(P<0.01)。结论MMP-9ASODN在体外能够显著降低A549细胞的黏附及迁移能力,有效地抑制肺癌A549细胞的增殖,同时促进其凋亡。
Background and objective Matrix metalloproteinase-9 （MMP-9） is one of significant extracellular matrix catabolic enzymes, which is known to relate to the initiation, progression and growth of the tumor. The aim of this study is to investigate the effects of MMP-9 antisense oligonucleotide （ASODN） on the apoptosis and metastasis of human lung adenocarcinoma A549 cell. Methods MTT was used to analyze the effect of MMP-9 ASODN transfection on A549 cell growth. Flow cytometry was used to analyze the cell cycle and apoptosis. MTT chromometry and scratch migration were used to observe the capability of adhesion and immigration of the A549 cell. Results MMP-9 ASODN inhibited the survival rate of the A549 cell and the action was concentration- and time-dependent. The peak concentration of MMP-9 ASODN was 600 nmol/L at 48 hours after transfection. After transfection of MMP-9 ASODN to A549 cell, the percentage of A549 apoptosis cell was significantly higher than that of control group （P〈0.01）, but the adhesion and migration cut down predominantly （P〈0.01）. Conclusion MMP-9 ASODN can depress the adhesion and migration and inhibit proliferation of adenocarcinoma A549 cell effectively, and can promote apoptosis of the A549 cell.
出处
《中国肺癌杂志》
CAS
2007年第5期351-355,共5页
Chinese Journal of Lung Cancer
基金
湖南省科技厅资助(No.06SK3029-7)~~
关键词
基质金属蛋白酶-9
反义寡核苷酸
A549细胞
凋亡
Matrix metalloproteinase-9 Antisense oligonucleotide Human lung adenocarcinoma cell Apoptosis
