摘要
目的:建立具有人免疫学特性的肾癌SCID小鼠模型。方法:SCID小鼠腹腔注射人外周血淋巴细胞,皮下接种人肾癌细胞,观察小鼠的生物学及免疫学特性。结果:(1)免疫重建荷人肾癌组小鼠成瘤率为100%,较荷瘤组成瘤潜伏期显著延长、肿瘤体积明显缩小(P<0.01);(2)第2、4、6周时小鼠外周血中人IgG分别为(394.86±16.70)μg/ml、(629.83±35.03)μg/ml、(994.96±70.11)μg/ml;(3)第6周小鼠外周血中人CD3+T淋巴细胞为(12.31±0.86)%;(4)免疫组化检测小鼠肿瘤和脾脏中存在人CD3+T淋巴细胞。结论:成功建立免疫重建荷人肾癌SCID小鼠模型,为肾癌治疗的研究提供了理想动物模型。
Objective：To investigate the feasibility of establishing a human renal cell carcinoma model in human peripheral blood lymphocyte-engrafted to severe combined immunodeficient （ hu-PBL-SCID ） mice. Methods ： The biological and immunological features of mice were evaluated after intra-peritoneal injection of human peripheral blood lymphoeytes （PBL） and subcutaneous implantation of human renal cell carcinoma cells （RCCCs）. Results.（1） Subcutaneous tumors developed in all the mice given human PBL and RCCCs. The latency period was significantly prolonged, and the tumor size was markedly depressed,as compared with the mice given RCCCs（P〈0.01）. （2） In the 2^nd,4^th and 6^th week,human IgG in the murine peripheral blood of the hu-RCCC-PBL-SCID model were （394.86 ± 16.70） μg/ml,（629,83 ± 35.03） μg/ml and （994.96 ± 70.11 ） μ g/ml, respectively. （3） In the 6^th week the human CD3^＋T lymphocytes was （ 12.31 ± 0.86）%, （4） Immunohistoehemieal staining revealed presence of remarkable human CD3CF lymphoeytes in murine tumor and spleen. Conclusion： A human renal cell carcinoma model has been established in hu-PBL-SCID mice which is an ideal animal model for preelinieal research and treatment for renal cell carcinoma.
出处
《重庆医科大学学报》
CAS
CSCD
2007年第11期1143-1146,共4页
Journal of Chongqing Medical University
基金
重庆市卫生局项目(编号05-2-024)
关键词
肾癌
SCID小鼠
免疫重建
动物模型
Renal cell carcinoma
SCID mice
Immune reconstructon
Animal model
