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RIG-Ⅰ与干扰素信号通路的关系 被引量：1

Relationship between RIG-Ⅰ and IFNs signaling pathway
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摘要 目的研究干扰素(IFNs)对维甲酸诱导Ⅰ型基因(RIG-Ⅰ)的调控作用,同时探讨RIG-Ⅰ对IFNs生物学效应的介导作用。方法Northern杂交和半定量RT-PCR检测小鼠胚胎成纤维细胞(MEFs)、在IFNs作用前后RIG-Ⅰ基因的表达。MTT比色法和细胞病变抑制法比较野生型和RIG-基因剔除(RIG-Ⅰ-/-)小鼠的MEFs在IFNs作用前后的生长情况和抗病毒活性。结果IFN-γ、IFN-β均能上调小鼠MEFs中RIG-Ⅰ的表达;100 U/mL IFN-γ处理后,野生型小鼠MEFs的生长抑制较RIG-Ⅰ-/-小鼠的MEFs明显;RIG-Ⅰ缺失后,IFN-β对细胞病毒感染的保护作用较野生型明显减弱。结论RIG-Ⅰ是一新发现的介导干扰素细胞效应的基因,它可能通过调节细胞因子的产生参与干扰素介导的炎症反应。 Objective To study the regulatory effect of interferons （IFNs） on retinoic acid-inducible gene-Ⅰ （ RIG-Ⅰ ） and the roles of RIG-Ⅰ in IFNs signaling pathway. Methods RIG-Ⅰ expression before and after IFNs treatment in mouse embryonic fibroblasts （MEFs） were analyzed with Northern blotting and semi-quantitative RT-PCR assay. MEFs isolated from wild-type and RIG-Ⅰ^-/- mice were used to test growth inhibition and antiviral activity of IFNs with MTT assay and cytopathic effect inhibition assay. Results Both IFN-γ, and IFN-β could induce RIG-Ⅰ expression in MEFs. Treated with 100 U/mL IFN-γ, growth inhibition and antiviral activity of MEFs from wild-type mice were more significant than those from RIG-Ⅰ^ -/- mice. With the absence of RIG-Ⅰ , the antiviral protective role IFN-β plays was significantly weaker than the wild type. Conclusion RIG-Ⅰ gene is a novel mediator of interferon effects on cells. It may participate in the inflammation responses mediated by IFNs through modulating cytokines production.
作者 孙岳平 肖家祁 邹嫣琼 王铸钢
机构地区 上海交通大学基础医学院细胞生物学教研室 上海交通大学基础医学院病原生物学教研室 上海交通大学基础医学院医学遗传学教研室
出处 《上海交通大学学报（医学版）》 CAS CSCD 北大核心 2007年第10期1210-1214,共5页 Journal of Shanghai Jiao tong University:Medical Science
关键词 维甲酸诱导Ⅰ型基因 干扰素 炎症反应 retinoic acid-inducible gene-Ⅰ interferons inflammation responses
分类号 R33 [医药卫生—人体生理学]
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上海交通大学学报（医学版）
2007年 第10期

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