摘要
以可自动恒温控温的锰锌铁氧体磁性纳米粒子(MZF-NPs)为核心,在其表面修饰聚乙烯亚胺(PEI)以制备一种新型纳米基因载体.利用扫描电镜、红外光谱仪、Zeta电位仪对其形貌、表面包覆功能团、电位等进行表征;UV/vis光谱仪及DNA体外结合、释放、转染实验研究了PEI在MZF-NPs上的定量吸附与修饰效果.电镜下修饰后粒子之间的聚集明显减轻、等电点由pH7.0移至pH11.5.PEI质量、介质的pH值以及离子强度均可影响PEI在锰锌铁氧体上的吸附.不同的吸附量也影响了纳米粒子的DNA结合、释放与转染能力.
Mn-Zn ferrite magnetic nanoparticles （MZF-NPs）, obtained from modified co-precipitation method, were surface-modified by polyethylenimine （PEI）. The appearance, function groups and charge of the surface were characterized by scanning electron microscopy （ SEM）, Fourier transform infrared spectroscopy （FTIR） and Zeta potential measurements. Adsorption behavior of PEI on MZF- NPs was measured by using UV/vis light Spectrophotometer. After modification, PEI-coated MZF-NPs show less agglomeration and the IEP changes from pH 7.0 to pH 11.5. The amounts of the immobilized PEI at the interface depend on PEI concentration, pH value and ionic strength of the media. The maximum amount of PEI absorbed on the MZF-NPs surface is 6.56 mg/g. The differences in PEI adsorption amount at the interface have consequences on the DNA binding ,release and transfection capacity of the PEI-coated MZF-NPs.
出处
《东南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2007年第5期867-872,共6页
Journal of Southeast University：Natural Science Edition
基金
国家自然科学基金资助项目(30500601
30371830)
国家高技术研究发展计划(863计划)资助项目(2002AA302207)
关键词
磁性纳米粒子
锰锌铁氧体
定量吸附
聚乙烯亚胺
magnetic nanoparticles
Mn-Zn ferrite
quantitative adsorption
polyethylenimine
