摘要
根据玉米细菌性枯萎病菌(Pantoea stewartiisubsp.stewartii)ITS基因序列,设计并合成了特异性PCR检测引物,对玉米细菌性枯萎病菌和非玉米细菌性枯萎病菌的标准菌株进行了PCR扩增反应.结果显示,玉米细菌性枯萎病菌的PCR产物出现301 bp的特异性扩增条带,而非玉米细菌性枯萎病菌均未出现扩增条带;其PCR检测的灵敏度为612 pg/μL.因此PCR技术是一种特异、灵敏、快速检测玉米细菌性枯萎病菌的方法.
A rapid identification method based on artii was established by using one pair of primers uni gene segments. A specific DNA amplicon was present molecular biology of Pantoea stewartii subsp, stewque to ITS（16 S-23 S rRNA interspacer region） in the standard strains,but absent in the non-stand strains,which showed that one pair of primers are a rapid molecular biological identification method of Pantoea stewartii subsp, stewartii. The sensitiv approximately 104,and the level of detection of ity th of this method was confirmed by serial dilutions while e primer was 612 pg/μL DNA solution.
出处
《甘肃农业大学学报》
CAS
CSCD
2007年第5期88-91,共4页
Journal of Gansu Agricultural University
基金
国家质检总局植物病原检测专项基金(2005IK066)
关键词
PCR
玉米细菌性枯萎病菌
检测
PCR
Pantoea stewartii subsp, stewartii
identification
