摘要
目的:研究循经温度形成的现象与Ca2+、Mg2+-ATP酶活性变化的关系,探讨循经高温线形成的能量代谢机制。方法:选用健康成年家兔7只,用红外热像仪观察温灸左后肢外侧"后三里"、右后肢内侧"阴陵泉"穴诱发胃经和脾经出现的温度变化。选取温灸后循经出现温度较高部位(高温区)的组织,用酶学方法测定Ca2+、Mg2+-ATP酶活性,并与非高温区(对照区)组织比较。结果:温灸家兔"后三里""阴陵泉"穴循经组织温度明显升高。右后肢内侧(足太阴脾经)高温区组织Mg2+-ATP酶活性明显高于对照区组织(P<0.05);左后肢外侧(足阳明胃经)高温区组织Mg2+-ATP酶活性亦有增高趋势,但与对照组比较差异无显著性意义(P>0.05)。温灸后,双后肢循经高温区组织Ca2+-ATP酶活性较对照区有升高趋势,但差异无显著性统计学意义(均P>0.05)。结论:1)家兔足太阴脾经循经出现温度较高组织的Mg2+-ATP酶活性较非高温区增强,可能导致外周ATP释放能量的功能活动加强,从而促使组织能量代谢旺盛。2)家兔循经温度较高组织的Ca2+-ATP酶活性未见明显改变,提示Ca2+-ATP酶活性在循经高温区的形成过程中可能不起主要作用。
Objective： To study the interrelation between the change of temperature and Ca^2＋ -, Mg^2＋-ATPase activity in the tissues along the running course of meridians in the rabbit. Methods： Seven healthy mongrel rabbits were used in this study. Before and after moxibustion of ＂Zusanli＂ （ST 36） and ＂Yinlingquan＂ （SP 9）, the cutaneous temperature along the Stomach Meridian and Spleen Meridian was detected with an infra-red thermography. The tissues showing higher temperature （including skin, subcutaneous tissue and muscle, 100 mg） and non-higher temperature （skin, subcutaneous tissue and muscle about 0.5 cm beside the higher temperature region, 100 mg） after moxibustion were sampled respectively for detecting the activity of Ca^2＋ - and Mg^2＋ - ATPase with enzymologic method. Results. After moxibustion of ＂Zusanli＂ （ST 36） and ＂Yinlingquan＂ （SP 9）, the temperature of the skin along the Stomach Meridian and Spleen Meridian on the same side increased significantly （P〈0. 05,0.01 ）; correspondingly, the activity of Mg^2＋ -ATPase in the higher temperature regions （lateral side of the left thigh, and the medial side of the right hindlimb） increased moderately and significantly separately in comparison with the control regions （P〈0. 05）. No marked changes were found in the activity of Ca^2＋ -ATPase in the same tissues of the higher temperature regions （ P〉0. 05）. Conclusion： In the tissues of higher temperature region after moxibustion of SP9, Mg^2＋ -ATPase activity increased evidently, that may contribute to the increase of temperature along the skin of the Spleen Meridian after moxibustion, while Ca^2＋ -ATPase may play a minor role therein.
出处
《针刺研究》
CAS
CSCD
2007年第5期330-333,共4页
Acupuncture Research
基金
"九五"国家攀登计划预选项目(No.95-预-19-313)
