摘要
目的探讨肿瘤坏死因子-α-1031位点基因多态性与冠心病的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测121例冠心病患者和115例正常人的TNF-α基因型。结果TNF-α- 1031位点基因型TT、TC+CC频率在患者组和对照组分别为66.9%、33.1%和64.3%、35.7%;等位基因T、C频率在患者组和对照组分别为82.6%、17.4%和80.9%、19.1%。两组基因型和等位基因频率比较差异没有显著性(P>0.05)。结论TNF-α-1031位点基因多态性与冠心病之间不存在明显相关性。
Objective To investigate the relation between tumor necrosis factor-α （TNF-α） -1031 position polymorphism and coronary heart disease in the Chinese population. Methods The polymorphism of TNF-α gene was screened by PCR-RFLP. Results The TNF-α-1031 genotype frequencies of TT, TC ＋ TC were 66.9% ,33.1% and 64.3% ,35. 7% in coronary heart disease and control group respectively. Allele frequencies of T, C were 82.6% ,17.4% and 80.9%, 19.1% respectively. There was no significant difference in frequencies of genotype and allele in TNF-α-1031 polymorphism between two groups （ P 〉 0. 05 ）. Conclusion No association between TNF-α polymorphism and coronary heart disease seems to exist in Chinese population.
出处
《公共卫生与预防医学》
2007年第5期9-11,共3页
Journal of Public Health and Preventive Medicine
关键词
肿瘤坏死因子-Α
基因多态性
聚合酶链反应-限制性片段长度多态性
冠心病
TNF-α
gene polymorphism
Coronary heart disease
Polymerase chain reaction-restriction fragment length polymorphism
Coronary heart disease
