摘要
目的比较观察脱氧胆酸钠体外对自然生长条件下的新生隐球菌新生变种(CZYD7)胞外磷脂酶的抑制作用。方法在37℃下,用蛋黄平板法培养并测量菌株产生沉淀圈的大小,用沉淀圈比值(PZ值)比较磷脂酶活力的变化。结果对照组与脱氧胆酸钠不同浓度组(依次为0.1%、0.2%、0.3%和0.4%)的PZ值分别为0.390±0.050,0.782±0.038,0.875±0.074,0.914±0.048,0.885±0.091;脱氧胆酸钠各浓度组与对照组比较均有显著差异(P<0.01)。脱氧胆酸钠不同浓度组间PZ值比较,0.1%和0.2%、0.1%和0.3%、0.1%和0.4%组间有显著差异(P<0.05)。结论脱氧胆酸钠体外对新生隐球菌新生变种CZYD7株胞外磷脂酶具有抑制作用。
Objectives To confirm the effect of sodium deoxycholate on the activity of phospholipases secreted by Cryptococcus neoformans vat. neoformans in vitro . Methods High phospholipase-producer Cryptococcus neoformans strain CZYD7 was chosed as subject. After being incubated onto egg yolk agar at 37℃ ,phospholipase activity of the strain was measured and compared by the ratio of the diameter of the colony to the total diameter of the colony plus precipitation zone （PZ）. Results In vitro , the PZs of control group and sodium deoxycholate groups in different concentration were 0. 390 ± 0. 050, 0.782 ± 0. 038, 0. 875±0.074, 0.914 ± 0. 048, 0. 885 ± 0.091 respectively ; PZs were significant different between the sodium deoxycholate groups of 0.1% ,0.2% ,0.3% and 0.4% and control group （ P 〈 0.01 ） ; There was significant difference between the sodium deoxycholate groups of 0.1% and 0. 2% ,0.3% ,0.4% （ P 〈 0.05 ）. Conclusions Sodium deoxycholate had an inhibition activity of phospholipases secreted by Cryptococcus neoformans var. neoformans strain CZYD7 and there was a correlation between the effect and concentration in vitral tests.
出处
《中国真菌学杂志》
2007年第5期264-266,共3页
Chinese Journal of Mycology
关键词
脱氧胆酸钠
新生隐球菌新生变种
磷脂酶
sodium deoxycholate
Cryptococcus neoformans var. neoformans
phospholipase
