摘要
乙烯是一种以气体状态存在的植物激素。通过在培养基中附加AgNO_3、KMnO_4和ACC初步研究了乙烯对滨梅生根的影响。结果表明低浓度的AgNO_3(1.0mg/L)显著促进了不定根的生长,根长平均为(2.39±0.30)cm,随着AgNO_3含量的递增,根的生长有受到抑制的趋势;当有KMnO4存在时,外植体平均生根条数与对照没有显著差异,但显著促进了根的生长,根均长为(6.30±0.48cm),与对照(4.88±0.32cm)有显著差别(p=0.028<0.05),可见乙烯积累对滨梅根的生长有显著抑制作用,但对根的诱导数量作用不明显;随着ACC含量的递增,每个外植体的生根数量与根的生长都受到抑制,与未加ACC相比,差异显著,说明ACC对滨梅根的诱导与生长起着负面的影响;高浓度的ACC诱导了外植体过早衰老,严重抑制了植物的生长发育。
The relationship between ethylene and adventitious root formation in beach plum in vitro culture was studied, and the effects of ethylene inhibitors （ silver nitrate （ AgNO3 ）, ethylene removal by KMnO4, and the ethylene precursor 1-aminocyclopropane-l-carboxylic acid （ACC）） on rooting were also tested in this paper. The results showed that low concentration of AgNO3 （ 1, 0 mg/L） significantly promoted root growth （ root length 2, 39 ±0. 30 cm）, and high concentration would inhibit root growth. Removing ethylene by KMnO4 from the headspace markedly also improved root growth （ root length 6. 30 ±0. 48 cm）, the average root length was significantly greater than the control, although the root number of per shoot was not significant difference compared with the control. Both root number and growth of explants were inhibited with the ACC added at 0. 1- 10 mg/L,indicating that ACC played a negative role on root induction and growth of beach plum.
出处
《林业科技开发》
2007年第6期67-70,共4页
China Forestry Science and Technology
基金
"十一五"国家科技支撑计划(编号:2006BAD09A04
2006BAD09A08)
