摘要
目的观察抗氧化剂普罗布考对颈动脉内膜切除术后早期再狭窄形成过程的影响,并探讨其作用机制。方法采用血管内膜气体损伤结合高脂饲料喂养的方法建立新西兰兔颈动脉粥样硬化动物模型,在此动物模型基础上行颈动脉内膜切除术,实验组在术前14 d开始用普罗布考胃管灌入并设立对照进行比较,采用病理及免疫组化等方法,观察术后早期再狭窄过程中普罗布考对细胞凋亡和细胞间粘附分子-1(ICAM-1)、基质金属蛋白酶-2(MMP-2)表达的影响。结果抗氧化剂普罗布考可有效地减少颈动脉内膜切除术后新内膜厚度,增加术后血管残腔的面积,减少血管平滑肌细胞的增殖和迁移,在此过程中普罗布考可上调Bcl-2的表达,抑制新内膜发生的凋亡,抑制颈动脉内膜切除术后ICAM-1、MMP-2的表达。结论抗氧化剂普罗布考抗再狭窄的作用机制与抑制颈动脉内膜切除术后ICAM-1、MMP-2的表达,减少新内膜的凋亡有关。
Objective To observe the effects of the antioxidant probucol on early restenosis after carotid endarterectomy （CEA） and its mechanism. Methods Carotid atherosclerotic models of New Zealand rabbits were established by air-drying plus high-cholesterol diet feeding, and CEA was performed on these animal models. Fourteen days before CEA, rabbits of the experimental group were gavaged with probucol through the gastric tube and a control group was established for comparison. Pathological and imrnunohistochemical techniques were applied to detect the effects of probucol on apoptosis and expressions of intercellular adhesion molecule-1 （ICAM-1） and matrix metalloproteinase-2 （MMP-2） during early restenosis following CEA. Results The antioxidant probucol could effectively reduce the neointima thickness after CEA and proliferation and migration of vascular smooth muscle cells （VSMCs）, increase postoperative area of vascular residual cavity, upregulate the expression of Bcl-2, suppress apoptosis in the neointima and inhibit the expression of ICAM-1 and MMP-2 after CEA. Conclusion The antioxidant probucol can inhibit restenosis after CEA possibly through decreasing expressions of ICAM-1 and MMP-2 and the apoptosis of the neointima.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第11期1138-1142,共5页
Chinese Journal of Neuromedicine
