摘要
目的:探讨HSPC300蛋白与原发性结肠癌发生与发展的关系,及其异常表达的细胞生物学意义。方法:用免疫组织化学方法分析HSPC300蛋白在正常、腺瘤及结肠腺癌中的表达;通过体外细胞迁移系统观察异常表达的HSPC300对结肠癌细胞迁移能力的影响。结果:HSPC300在正常结肠组织中的表达阳性率为25%(5/20),在结肠腺瘤组织中为15%(3/20),结肠癌组织中为68%(15/22)。HSPC300在结肠正常组织及腺瘤组织中的表达频率显著低于腺癌(P=0.0002)。体外迁移实验表明,下调结肠癌细胞中HSPC300的表达可降低其迁移能力。结论:HSPC300在结肠癌的发生、发展过程中是一个晚期分子事件,其高表达有助于结肠癌细胞获得转移潜能。
Objective： To study the expression distribution of HSPC300 in colonic normal, adenoma and carcinoma tissues, and migration of carcinoma cell in vitro. Methods： By tisuue immunochemistry, the expression of HSPC300 in colonic normal, adenoma and carcinoma tissues was detected. By the system of cell migration in vitro, the effect of abnor- mally expressed HSPC300 to the migration of colonal cancer cell was also observed. Results： The results showed that ex- pression of HSPC300 elevated significantly in colon carcinoma tissue, compared with those in normal and adenoma tissue. Down-regulated expression of HSPC300 by siRNA would inhibit the migration of colonal cancer cell in vitro. Conclusion： It indicated that the up-regulated expression of HSPC300 may contribute to the progress of colonal cancer.
出处
《生物技术通讯》
CAS
2007年第6期924-926,共3页
Letters in Biotechnology
