摘要
应用电镜超薄切片技术，观察福建省四个主要养鳖市县现场采集的鳖出血病样品．首次发现了一种感染养殖中华鳖的球形病毒（Ｔｒｉｏｎｙｘｓｉｎｅｎｓｉｓｓｐｈｅｒｏｖｉｒｕｓ，ＴＳＳＶ）．该病毒分布在病鳖的肺、胃、咽喉粘膜和腹甲皮层．主要靶细胞是这些组织的小血管和毛细血管的内皮细胞．ＴＳＳＶ粒子近球形，直径３５～３９ｎｍ，无囊膜包被，有的成群聚集在内皮细胞质中，有的由单位膜包裹在包涵体中．病毒感染的内皮细胞病变明显．还讨论了ＴＳＳＶ与出血病的关系．
The biological characterstics of A and B type of Crithidia bombi from multiple infection was analyzed.It was found that there were variation in the number of the cell binary fission and the time for establishment of clones between the two types.After 3 days of cloning,the mumber of the binary fission were from 3 to 6 times in type A, and from 1 to 6 times in type B.For the time for establishment of clones (the cells up to 10 7/ mL in 200 μL),11 to 16 days needed for clones in type A,and 10 to 21 days are needed for that in type B. The reaults implied that there was competition between these two types under multiple infection. It was also found that there was variationin the body shape and size of the cells in two types and the variation could be used as anuimportant genetic marker in the dynamic studies on the competition of two types in the same host.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第4期626-630,共5页
Journal of Xiamen University：Natural Science
基金
福建省水产厅资助
