摘要
目的探讨黄芪对慢性乙型肝炎(chronic hepatitis B,CHB)患者外周血Th1/Th2功能平衡的影响。方法CHB患者和健康人的外周血单个核细胞体外经黄芪作用,培养48h后,用双抗体夹心酶联免疫吸附法检测培养上清中Th1类细胞因子(IL-2、IFN-γ)和Th2类细胞因子(IL-4、IL-10)的含量变化。结果在黄芪诱导下:①健康对照组和CHB患者组Th1类细胞因子明显增高(P<0.01),Th2类细胞因子水平无明显变化(P>0.05);②CHB患者组IFN-γ水平明显高于健康对照组(P<0.01),IL-2水平两组比较差异无统计学意义(P>0.05);③Th2类细胞因子水平CHB患者组比健康对照组高,差异有统计学意义(P<0.01)。结论黄芪可明显提高CHB患者Th1类细胞因子(IFN-γ、IL-2)的产生水平,改善CHB患者Th1/Th2功能失衡状态,可使CHB患者Th2优势应答状态部分得到纠正。
Objective To study the effects of AM on the production of Th1/Th2 cytokines by peripheral blood mononuclear cells from chronic hepatitis B （CHB） patients. Methods Peripheral blood mononuclear cells （PBMCs） from normal and CHB patients were collected and stimulated in vitro with AM for 48 hours. The levels of IL-4, IL-10, IL-2, and IFN-γ in the culture supernatants were determined by sandwich enzyme-linked immunosorbent assay （ELISA）. Results AM stimulated the production of Thl but not Th2 cytokines by PBMCs from normal control and CHB patients （P〈0.01）. AM stimulated the production of IFN-γ by PBMCs from CHB patients （P〈0.01）. The level of IL-2 between the two groups was not changed （P〉 0.05 ）.The levels of Th2 cytokines in CHB patients are lower than the normal control （P〈0.01）. Condusion AM was found to significantly increase the production of Thl like cytokine by PBMCs from CHB patients. AM may enhance the Thl function and maintaine the balance of Th1/Th2 in CHB patients.
出处
《热带医学杂志》
CAS
2007年第11期1088-1090,共3页
Journal of Tropical Medicine
基金
广州市中医药中西医结合科研课题(No.2005B050)
广州市海珠区科技局课题(No.2006-D-21)。
