摘要
A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.
基于抗体的酶连接了的高亲密关系 polyclonal 免疫吸着剂试金(ELISA ) 在牛的尿为 zeranol 的数量化离子被开发。根据尿矩阵研究，生产不足道的矩阵干扰的优化冲淡因素在预告的处理作为 1:5 被选择。在改进 ELISA，线性反应范围在 0.02 之间；1 μ g /ml，；察觉限制是为试金的 0.02 μ g /ml。全面恢复；变化(CV ) 的系数在 82%～ 1 的范围 27% ；3.5%～ 8 .8% 分别地。与 zeranol 刺的 36 件牛的尿样品(从 0.2 ～ 10 μ g /ml ) 被 ELISA 检测；液相色层分析法(LC ) 方法，；好关联在二个方法之间被获得(R [2 ]=0.9643 ) 。我们断定这改进 ELISA 是为屏蔽的集体 zeranol 的合适的工具；能是为为在牛的尿的 zeranol 的常规 LC 方法的一种选择。
基金
Project supported by the National Natural Science Foundation of China (No. 30471155)
the Agriculture Key Technologies R & D Program of Shanghai (No. (2003) 9-4), China
