摘要
目的:联合检测类风湿关节炎(RA)患者血清中的抗环瓜氨酸抗体(CCP)和抗角蛋白抗体(AKA)、抗核周因子抗体(APF),探讨RA的早期诊断方法。方法:对已确诊的71例RA患者、207例非RA的自身免疫病患者同时测定抗CCP抗体(ELISA法)和AKA、APF(间接免疫荧光法)。结果:AKA、APF及抗CCP抗体对早期RA的敏感性分别为46.5%、77.5%和69.0%;特异性分别为100%、100%和98.8%。CCP及APF的阳性检出率高于AKA(p<0.01)而与类风湿因子(RF)无差异(p=0.067).RF弱阳性或阴性的RA患者,AKA、APF及CCP可为阳性。结论:CCP抗体及APF对RA具有较好的敏感性和高度的特异性,联合检测AKA、APF、CCP抗体可作为早期RA病人及RF阴性RA病人的早期诊断指标。
Objective： To exam the anti -cyclic citmUinated peptide antibody （CCP）, anti -keratin antibody（AKA） and antipefinuclear factor（APF） and to evaluate the diagnosis method of the rheumatoid arthritis （RA） . Method： Serum level of CCP, AKA and APF were examed simultaneously by ELISA and indirect immunofluorescence（IIF） respectively in 71 RA and 207 non -RA patients. Result： In RA patients, the sensitivity of anti -CCP, AKA and APF were 46.5% ,77.5% and 69.0% ,while the specificity were 100% ,100% and98.8% respectively. As for sensitivity, there was a significant difference between the methods of anti - CCP, APF And AKA assay . However , no difference was found between anti - CCP, APF And RF assay . The AKA, APF and anti - CCP can be positive in the RF positive RA patients and the negative RA patients. Condusion： Anti - CCP and APF is quite sensitive and specific in the diagnosis of RA. To exam the anti -CCP, AKA and APF may be useful factors for early positive or negative RA patients.
出处
《河北医学》
CAS
2007年第5期509-512,共4页
Hebei Medicine
