摘要
以韭菜种子萌发后根尖及继代组培苗根尖材料为外植体,以MS为基本培养基附加不同种类和浓度的植物激素诱导产生愈伤组织及芽。研究表明BA、KT和NAA配合使用利于根尖材料芽分化,种子根尖比继代组培苗根尖诱导愈伤形成及芽分化相对容易,芽分化率达80%,经过根的预培养,继代组培苗根尖可达到60%～65%的芽分化率。
An efficient plant regeneration system was established through optimization of MS with different hormone combination and different type of root from seed and subcuhured seedling in Chinese chive. Result showed the combination of BA,KT and NAA was suit for root tip shoot differentiation, the root tip of seed was easier to form callus and shoot than the subcuhured seedling, the shoot differentiation rata was 80%, through special culture methods, that of the subcultured seedling root tip could reach 60%～65^.
出处
《北方园艺》
CAS
北大核心
2007年第12期199-200,共2页
Northern Horticulture
关键词
韭菜
根尖
愈伤组织
芽分化率
Chinese chive
Root tip
Callus
Shoot differentiation rates
