摘要
目的探讨海生素对K562/ADM细胞的抑制作用及其对凋亡相关蛋白bcl-2、bax表达的影响。方法采用MTT法测定海生素对K562/ADM细胞的体外抑制作用,免疫细胞化学法检测bcl-2、bax蛋白的表达。结果海生素对K562/ADM细胞有抑制作用,可下调bcl-2蛋白表达,上调bax蛋白表达。结论海生素在体外能明显抑制K562/ADM细胞生长,并影响bcl-2、bax蛋白的表达。
Objective： To study the inhibitory action and the effect on apoptosis associated protein on K562/ADM cells by Haishengsu. Methods： The procedure of MTY assay was used to determine the inhibitory action on k562/ADM cells, and immunocytochemistry was used to detect the expression of bcl - 2 and bax protein. Results ： Haishengsu could inhibit the proliferation of K562/ADM cells, and could down - regulate the expression of bcl - 2 protein and up - regulale the expression of bax protein on k562/ADM ceils. Conclusion ： Haishengsu can obviously inhibit the proliferation of K562/ADM cells and affect the expressions of bcl- 2 and bax protein.
出处
《泰山医学院学报》
CAS
2007年第4期279-281,共3页
Journal of Taishan Medical College
