摘要
目的:建立精浆尿酸(UA)的检测方法并探讨精浆UA水平与精液参数的相关性。方法:根据检测血清UA的方法加以改良建立检测精浆UA的方法,并观察批内变异和不同技术人员检测结果之间的差异以评价方法的可接受性。同时分析138例男性精浆UA水平与患者年龄、禁欲时间、精液量、pH、精子密度、活动率、a+b级精子百分率和正常形态精子百分率之间的相关性。结果:精浆UA检测方法的批内变异为9.16%,2位技术人员的检测结果没有显著性差异(P=0.541)。精浆UA水平与正常形态精子百分率呈正相关(r=0.350,P=0.025),与精子活动率和a+b级精子百分率有呈正相关的趋势(r=0.147和0.156,P=0.085和0.068),而与精液分析其他参数如精液量、pH、精子密度、禁欲时间以及患者年龄等无相关性。结论:通过对血清UA检测方法加以改良可以建立可接受的精浆UA检测方法。精子形态学、活动率及a+b级精子百分率可能与精浆UA水平高低有关。
Objective： To establish the method of seminal uric acid （UA） determination and investigate the correlation between the seminal UA level and semen parameters. Methods： The method of seminal UA determination was established by modifying the method of serum UA detection, and its intraassay coefficient of variation （CV） and the difference of the results between different technicians were also observed to evaluate the acceptability of the method. In the meanwhile, the correlations of the seminal UA level with the patients＇age, abstinence time, semen volume, pH, sperm concentration, motility, the percentage of grade a and b sperm, and the percentage of morphologically normal sperm. Results： The intraassay CV was 9.16% for the method of seminal UA detection, and there was no significant difference in the UA level detected by 2 technicians （ P = 0.541 ）. The seminal UA level was positively correlated with the percentage of morphologically normal sperm （ r = 0. 350, P = 0. 025 ）, with a tendency of positive correlation with sperm motility （ r = 0. 147, P = 0.085 ） and the percentage of grade a and b sperm （ r = 0. 156, P = 0.068 ） , but not with other parameters such as semen volume, pH, sperm concentration, abstinence time and the patients＇age. Conclusion： An acceptable method of seminal UA determination could be established by modifying the method of serum UA detection. Sperm morphology, motility and the percentage of grade a and b sperm might be related to the level of seminal UA.
出处
《中华男科学杂志》
CAS
CSCD
2007年第11期1016-1019,共4页
National Journal of Andrology
关键词
尿酸
精浆
精液参数
检测
uric acid
seminal plasma
semen parameter
determination
