摘要
目的：制备ｒｈＧＣＳＦ的１２５Ｉ标记物，研究其大鼠的药代动力学。方法：采用氯胺Ｔ氧化法制备１２５ＩｒｈＧＣＳＦ，并利用其对大鼠进行静注３种剂量的药物代谢动力学试验。结果：１２５ＩｒｈＧＣＳＦ放射性比度为１３９１２０ＧＢｑ／ｍｍｏｌ，放射化学纯度（ＲＣＰ）大于９５％。药物动力学研究表明，药时曲线符合二室模型，Ｔ１／２α分别为０．２２，０．３０和０．４４ｈ，Ｔ１／２β分别为７．７９，８．７６和８．１５ｈ。结论：为临床上安全合理用药提供依据。
Objective:Radioiodine labelled recombinant human granulocyte colony stimulating factor (rhG CSF) was prepared to study its distribution,excretion,metabolism and pharmacokinetics in rats.Methods: 125 I rhG CSF was prepared using chloramine T oxidation method and the pharmaceutical metabolism test of 125 I rhG CSF was studied in rats by intravenous injection in three doses.Results:Radiospecific activity of 125 I rhG CSF product had 139 120 GBq/mmol and radiochemical purity (RCP) had a higher 95%.Medicine time curves conformed to two room model. T 1/2 α and T 1/2 β had 0.22,0.30.0.44 h and 7.79,8.76,8.15 h separately.Conclusion:The basis of safe and reasonable given medicine is clinically provided in this study.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第2期120-123,共4页
Academic Journal of Second Military Medical University
关键词
RHG-CSF
集落刺激因子
碘125标记
药代动力学
recombinant human granulocyte colony stimulating factor
radioiodine labelling
metabolism
pharmacokinetics
