摘要
目的研究鲍曼不动杆菌耐药机制和流行病学特性。方法通过药敏试验、耐药基因检测、三维试验、膜外排机制试验进行耐药机制研究,采用扩增片段长度多态性分析(AFLP)技术研究耐药鲍曼不动杆菌之间的差异,通过聚合分析研究耐药菌之间的同源性。结果根据药敏分析、耐药基因分析和AFLP分析的结果,本研究发现三者具有一致性。多基因聚合分析发现存在至少3个克隆。AFLP分型发现耐药菌是由同一个克隆进化而来。结论医院的ICU病房已经成为耐药AB菌发生、进化、传播的核心环节。加强ICU的管理工作是防止院内耐药AB菌流行的重要手段。
Objective The study analyse the multi - drug resistance and epidemiologieal investigation in Acinetobacter baumannii. Methodes Using, multi -drug resistance gene test, three -dimension test, membranes active efflux test studied multi -drug resistance and epidemiologieal investigation. The genetype were analyzed by Amplified Fragment Length Polymorphism（AFLP）. The strains＇ homology were researched by multi - gene cluster analysis methods. Results Cluster analysis show that the result of drugsensitive test, multi - drug resistance gene test and AFLP are accordant. The study find at least 3 clones by multi - gene cluster analysis methods. AFLP show that all of the multi - drug - resistance of A. baumannii evolutes from a clone. Conclusions The study combined with clinical practice find that ICU in Ningbo No. 2 Hospital now is the core of the arises, evolution, transmission of A. baumannii. Strengthening management of ICU is very important to pretect the prevalence of Acinetobacter baumannii in nosocomial infection.
出处
《医学研究杂志》
2007年第12期29-32,共4页
Journal of Medical Research
基金
宁波市卫生局科研基金项目(2002053)
关键词
鲍曼不动杆菌
药敏试验
耐药基因
AFLP
同源性
Acinetobacter baumannii
Drugsensitive test
Drug - resistaneegene
AFLP
Relationship
