摘要
目的用壳聚糖和海藻酸钠为原料,制备干扰素-tau微囊。方法采用凝聚法制备了干扰素-tau壳聚糖-海藻酸钠微囊,以包封率、微囊强度为指标,设立总的评估指数,用L9(34)表进行正交实验优化制备工艺。结果最终的优化方案为:2.0%海藻酸钠、0.4%壳聚糖、0.3%干扰素-tau以及1.0%氯化钙溶液。结论该工艺简便、稳定,壳聚糖-海藻酸钠微囊有望用作干扰素-tau或其它蛋白质药物的口服制剂技术。
Purpose The microcapsules were prepared by using chitosan and alginate as raw materials, and the controlled-release microcapsules of interferon tau were hoped to develop an oral interferon preparation. Methods Interferon tau chitosan-alginate microcapsules were prepared by coacervation technology. Four factors and three levels for each factor were selected and arranged in an L9 （3^4） orthogonal experimental table. The evaluating indicator（EI% ）, calculated according to drug encapsulated efficiency（EE% ） and strength of microcapsules （MS % ）, was introduced as a total index for the microcapsules formulation. Results The best program is：2.0% sodium alginate, 0.4% chitosan, 0.3% IFN-tau and 1.0% CaCl2. Conclusion The preparation procedure is good, simple and reproducible. Chitosan-alginate microcapsules have the potential to be used in oral administration technology of interferon tau and the other protein medicines.
出处
《中国生化药物杂志》
CAS
CSCD
2007年第6期378-381,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
山东省科技厅资助(No.0310501010)
关键词
干扰素-tau
壳聚糖
海藻酸钠
包封率
微囊
interferon tau
chitosan
sodium alginate
encapsulation rate
microcapsules
