摘要
目的:探讨牡丹江市区三个人工湖(月牙湖、牛角湖、南湖)的微生物群落多样性。方法:从三个水样中分别提取微生物DNA,选取通用引物扩增16SrDNA片段,对扩增产物进行RFLP分析。结果:不同的样品由于其中的微生物多样性的差异,酶切产物在凝胶上显示的相对位置和条带数目都有一定差异。结论:牛角湖样品的条带数目最多,表明牛角湖的微生物群落多样性最为丰富。
Objective： The microbial community diversity of three artificial lake（Yueya lake, Niujiao lake, Nanhu lake） of Mudanjiang urban district were stuied. Methods： The total bacterial DNA from the three water samples was extracted directly. The fragments of 16S rDNA were amplified by the Polymerase Chain Reaction（PCR） and subsequently analyzed by RFLP. Results： The samples got differem bands because of the differenee of microbial diversity in the three lakes. Conclusion： The microbial community diversity of Niujiao Lake was in the richest.
出处
《生物技术》
CAS
CSCD
2007年第6期35-37,共3页
Biotechnology
