摘要
丁吡吗啉(pyrimorph)是一种结构新颖的杀菌剂,室内生物测定结果表明,其对致病疫霉Phytophthora infestans的菌丝生长、孢子囊产生、休止孢萌发具有较强的抑制作用,其EC50值分别为0.066、0.059和0.550μg/mL,但对游动孢子释放的抑制作用较弱,其EC50值为9.78μg/mL。显微镜下观察结果表明,经丁吡吗啉处理后的菌丝分枝相对较少,分枝间距拉长,但对菌丝直径无影响;从致病疫霉对番茄叶片的侵染过程来看,丁吡吗啉浓度为100μg/mL时,处理24h后在叶片表面只能看到少量休止孢萌发,96h后未产生孢子囊。丁吡吗啉在1μg/mL和10μg/mL时对菌丝体细胞膜的电导率无影响,但当浓度为50μg/mL时其电导率值明显上升;初步测定结果表明,在100μg/mL浓度下,其对致病疫霉菌丝体的蛋白质合成有一定的抑制作用,但对其菌丝体内的DNA合成没有明显的抑制作用。
Pyrimorph is a novel fungicide developed by China Agricultural University and Institute of Plant Protection. Bioassay of pyrimorph to Phytophthora infestans was conducted. The results showed that the toxicity of pyrimorph was high in controlling P. infestans. It could intensively inhibit the mycelium growth,sporangium production and cystospore germination of P. infestans and the EC50 value was 0. 066,0.059 and 0.550 μg/mL, respectively. It was insufficient in inhibiting the release of zoospores from sporangia and the EC50 value was 9.78 μg/mL. Microscopic observation of pyrimorph treatment showed that the mycelium branch was fewer and the space was longer than that of CK. The difference of hypha diameter was not distinct between pyrimorph treatment and CK. When P. infestans invaded inside tomato leaves,it showed that only a few cystospores germinated 24 hours after inoculation and there was no sporangium 96 hours after inoculation of pyrimorph treatment at 100 μg/mL. Pyrimorph was found to influence on the permeability of the cell membrane of P. infestans at 50 μg/mL, and the difference was not distinct at 1 μg/mL and 10 μg/mL. It showed that pyrimorph had some effect on the protein biosynthesis of P. infestans at 100μg/mL, but had little effect on the DNA biosynthesis.
出处
《农药学学报》
CAS
CSCD
2007年第4期376-382,共7页
Chinese Journal of Pesticide Science
基金
国家重点基础研究发展计划(973计划)(2003CB114403)
国家科技支撑计划项目(2006BAE01A03-10)
关键词
丁吡吗啉
致病疫霉
生物活性
作用机制
pyrimorph
Phytophthora infestans
biological activity
action mode
