摘要
基于主要农艺性状及AFLP分子标记技术，对40份入选菜用大豆品种进行遗传多样性评价，为拓宽菜用大豆种质资源基础及新品种选育提供理论依据。研究结果表明，基于农艺性状及品质性状的聚类将40份材料划分为3个类群；AFLP标记数据聚类将40个材料划分为3个AGs（AFLP—based groups）分子类群，且聚类结果与材料的地理来源无关。另外，8对AFLP引物共扩增出30条多态性条带。平均每对引物3．75个等位变异；品种间的遗传相似系数变化范围为0．3704～0．9998，平均值变化范围为0．5980～0．7924，总体平均值为0．7094，说明供试材料间的遗传相似系数较高。遗传差异较小，因此必须进一步加强菜用大豆种质资源的筛选和创新工作。
Genetic diversity of 40 vegetable soybean varieties was evaluated based on agronomic characters and AFLPs. The results showed that the 40 varieties could be divided into 3 groups according to the agronomic traits and AFLP polymorphic loci,respectively. Thirty DNA polymorphic bands were scored from amplification of 8 AFLP primer combinations, with 3. 75 markers for each primer combination in average. And the range of pair similarity coefficient was from 0. 370 4 to 0. 999 8; the average pair similarity coefficient was from 0. 598 0 to 0. 792 4 with the total average of 0. 709 4. The results also showed a narrow genetic diversity among the vegetable soybean varieties tested, thus, it is necessary to expand the germplasm resource of vegetable soybean.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2007年第4期391-396,共6页
Chinese Journal of Oil Crop Sciences
基金
河北省自然科学基金项目(C2004000351)
