摘要
目的分析海藻酸钠地塞米松凝胶(Alg-Dex凝胶)经圆窗给药后地塞米松在外淋巴液中的浓度变化。方法健康豚鼠30只随机分成5组,每组6只,于每只豚鼠单侧(右耳)经圆窗给海藻酸钠地塞米松凝胶(45mg/ml)20μl后的1、2、3、4、5天分别用高压液相色谱仪分析外淋巴液中的地塞米松浓度。结果在Alg-Dex凝胶给药后1、2、3、4、5天在外淋巴液中均能检测到地塞米松,浓度分别为0.49±0.06、1.32±0.28、0.65±0.08、0.66±0.05、0.53±0.17mg/L,其中第2天浓度最高(1.32±0.28mg/L),第3～5天药物浓度较稳定,平均浓度为0.61±0.07mg/L。结论海藻酸钠能作为地塞米松良好的缓释载体,使其缓慢、持续地经圆窗膜渗透到外淋巴液中。
Objective To determine pharmacokinetics profiles of sodium alginate dexamethasone in perilymph after trans--round window administration of the sodium alginate dexamethasone gel in vivo. Methods 30 healthy guinea pig were randomly divided into 6 groups. Alg--Dex gel was placed into the niche of the round window of the guinea pig on the right ear. The perilymphetic samples were harvested on day 1,2,3,4 and 5 after administration of Alg-- Dex gel respectively. The concentrations of dexamethasone in perilymph were assayed with high performance liquid chromatograph （HPLC）. Results Concentrations of dexamethasone in perilymph on day 1,2,3,4 and 5 after administration of Alg--Dex gel were 0. 49±0. 06,1. 32±0. 28,0.65±0. 08,0.66±0.05,0.53±0.17 mg/L respec- tively. Concentrations of dexamethasone in perilymph reached a peak on the second day and maintained 0.61± 0.07 mg/L steadily on following days after administration. Conclusion Dexamethasone can be released from the sodium alginate dexamethasone gel persisitently and steadily in vivo.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2008年第1期57-59,共3页
Journal of Audiology and Speech Pathology
基金
上海市科委基金资助项目(No:0341l9819)
