摘要
目的探讨中药青蒿琥酯(artesunate,AR)对脂多糖(LPS)诱导的RAW264.7细胞损伤状态下的保护作用及其机制。方法采用体外培养的巨噬细胞系RAW264.7细胞,待细胞生长至融合状态后分别加入LPS和不同浓度的AR(2.5,5,10,20μg/ml),于37℃、5%CO2条件下共同孵育24 h,设立空白对照组。采用反转录PCR(RT-PCR)技术检测细胞核因子κB(NF-κB)mRNA表达的变化,硝酸还原酶法检测细胞上清液中炎性介质一氧化氮(NO)的含量。结果1μg/ml的LPS刺激后,巨噬细胞信号转导通路上NF-κB的表达及炎症介质NO的含量均较对照组明显增加(P<0.05),加入AR后,随AR浓度的增加逐渐下调LPS升高的(NF-κB)mRNA表达及N0含量,且与LPS组比较差异有统计学意义(P<0.05)。结论青蒿琥酯可明显降低内毒素诱导的巨噬细胞NF-κB的表达,减少NO的分泌可能是其抗损伤的保护作用机制之一。
Objective To investigate the protective effects and the mechanism of artesunate（AR） on injury of RAW264.7 cells induced by endotoxin.Methods A macrophage cell line RAW264.7 was employed in an in vitro experiment.After RAW 264.7 cells were cultured and turned to fusion manner,LP5 and different concentration of AR（2.5,5,10,20μg/ml） were added respectively and co-incubated for 24hours at 37℃ with 5% CO2,and to establish blank control group.RT-PCR were used to observe nuclear fator κB（NFκB） mRNA expression.The content of inflammatory mediators NO in the conditioned media was tested by nitrate reductase.Results After being stimulated to lμg/ml LPS,NF-κB expression and the content of NO were higher than those in the control group on macrophage signal transduction pathway（P〈0.05）.AR could significantly down-regulate the increased NF-κB expressions and the content of NO concentration-dependently,as well statistical significance showed compare with LPS group（P〈0.05）.Conclusion One of the most important anti-damnification mechanism that artesunate could significantly decrease the quantity of NO by downing regulation the expression of NF-κB is the essential factor of LPS signal transduction pathway under LPS.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2008年第1期54-55,共2页
Chinese Journal of Public Health
基金
广东省自然科学基金(5004774)
关键词
青蒿琥酯
内毒素
信号转导
artesunate
endotoxin
signal transduction
