摘要
目的：测定不同产地的穿心莲药材中穿心莲内酯和脱水穿心莲内酯的含量。方法：采用高效液相色谱（HPLC）法,Merck-Lichrospher RP-C18柱（4.6mm×250mm,5μm）,流动相为甲醇-水（60∶40）;检测波长：226nm（用于检测穿心莲内酯）,254nm（用于检测脱水穿心莲内酯）;流速：0.8ml/min;柱温：35℃。结果：实验表明测定方法在4h内是稳定的,穿心莲内酯线性范围0.050～1.500μg,脱水穿心莲内酯线性范围0.048～1.440μg,平均加样回收率分别为100.9%和98.2%,RSD分别为3.4%（n=6）和3.2%（n=6）。结论：用HPLC法测定穿心莲药材中穿心莲内酯和脱水穿心莲内酯的含量方法准确、灵敏、稳定,可作为控制穿心莲质量的方法。
Objective：To investigate the contents of andrographolide and didehydro-andrographolide in Hebra androyraphis.Methods：HPLC was performed on Merck-Lichrospher RP-C_ 18（250 mm×4.60 mm,5 μm）.The chromatographic conditions were as follows：Methanal-Water（60∶40）as mobile phase,flow rate is 0.8 ml/min,detecting wave length at 226 nm（andrographolide）and 254 nm（didehydro-andrographolide）,column temperature was 35℃.Results：A good linearity of andrographolide was shown in range of 0.050～1.500 μg（r=0.999 9）,and that of didehydro-andrographolide was 0.048～1.440 μg（r=0.9999）,the average recovery rate were 100.9%（andrographolide）and 98.2%（didehydro-andrographolide）,there RSD were 3.4%（andrographolide）and 3.2%（didehydro-andrographolide）.Conclusion：this method can supply evidence for the equality evaluation of Hebra androyraphis.
出处
《江西中医学院学报》
2007年第6期40-41,共2页
Journal of Jiangxi College of Traditional Chinese Medicine
关键词
穿心莲
穿心莲内酯
脱水穿心莲内酯
含量测定
HPLC
Hebra androyraphis
Andrographolide
didehydro-andrographolide
Content Determination
HPLC
