摘要
目的探讨生物素葡聚糖胺(BDA)神经示踪剂在大鼠皮质脊髓束通路的神经示踪实验研究。方法采用成年的SD大鼠,用牙科钻开颅形成两个约为3mm直径的圆形骨窗,用微量注射器将15%的BDA缓慢注射入左侧运动感觉皮质区,每个骨窗选择3点3层注射,注射总量108μl。存活14d后,取出脑和脊髓组织在低温冰箱中保存,冰冻切片后用荧光显微镜观察BDA被轴突摄取后运输的情况。结果在大脑感觉运动皮质层注射BDA后,在注射大脑半球以及皮质脊髓束通路的中脑、桥脑、延脑和脊髓颈膨大处均可见荧光显影,放大200倍时能清楚看到轴突结构及郎氏结。结论BDA具有生物性稳定、转运距离远等优点,含自发荧光的BDA实验方法简单,为大鼠皮质脊髓束通路的形态学研究提供了可靠的技术平台。
Objective To explore the effects of biotin dextran amine （BDA） as a nerve tracer in rat corticospinal tract. Methods Adult SD rats were used in this experiment and 15 % BDA was injected slowly into the left sensory and motor cortex with a mini syringe through two round-shape bone windows with 3mm diameter, formed by a dental drill. Three spots were chosen for injection within each bone window in different layers,with the total volume 108μl. After survival for 14 days,brains and spinal cords were removed and stored in a freezer. Sections were made in a cryostat and transportation of BDA after absorption by axons were observed under a fluorescence microscope. Results The BDA injected into the left cortex is taken by the axon,and the pyramidal cells and the axon in the left cerebral cortex appeard the widespread fluorescence development ,with the time going on,the BDA taken by the cerebral cortex axon transfers to the midbrain and cervical enlargement along the CST with long distance, and structures of axon and nodes of ranvier could be observed clearly with a 200 × magnification. Conclusion Injecting the BDA into the cerebral cortex can trace the corticospinal tract effectively. It is biologically stable and can be transported for a long distance. The experiment using spontaneou fluorescence is simple and provides a reliable method for the morphological study of corticospinal tract of rats.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2007年第6期687-689,共3页
Journal of Apoplexy and Nervous Diseases
