摘要
高温放线菌是堆肥过程中微生态环境的重要组成成分,在好氧堆肥的升温期对有机物的分解发挥重要的作用。在造纸污泥堆肥过程的高温期分离到一株高温放线菌AO-Ⅲ,其形态和主要生理生化特征与高温放线菌属的典型种相似,但该菌株能耐受80℃以上的高温。菌株AO-Ⅲ16SrDNA序列经校对、拼接后与GenBank数据库中相关种的序列进行比对,并通过MEGA3构建进化树进行分析,该菌株与甘蔗兰希氏菌(曾用名:普通高温放线菌,登录号:AY114169.1)高度同源,同源率为99.9%,因此,菌株AO-Ⅲ鉴定为甘蔗兰希氏菌(Laceyella sacchari)。以该菌株为外源微生物添加到造纸污泥与味精废液的混合物中进行堆肥试验。结果表明,在高温好气性堆肥发酵过程中达到高温阶段(80℃以上)的时间由原来的3d缩短为1d,高温好气性发酵周期由20d缩短至10d,水份由55%降至40%以下。该菌株能够维持较长的高温期时间及缩短堆肥发酵周期。
Thermophilic actinomycetes are well known components of the microflora of composts and play an important role in habitats where decomposition of organic matter takes place at elevated temperatures and under aerobic conditions. A thermophilic actinomycete strain AO-Ⅲ was isolated from the composting process of pulp and paper mill sludge at the thermophilic stage. The main biochemical and physiological features, and morphological characters of this strain resembled those of some strains of species belonging to the genus ＇thermoactinomyces ＇, except AO-Ⅲ could grow aerobically at over 80 ℃ on a solid medium. The 16S rDNA gene sequences of AO-Ⅲ were aligned and corrected and then were compared with the corresponding sequences of standard strains obtained from the GenBank database to evaluate taxonomic position by using MEGA 3. The strain AO-Ⅲ showed highest similarity of 99.9% to LaceyeUasacchari （former name： Thermoactinomyces sacchari, accession numbers： AY114169.1）. So it was nominated LaceyeUa sacchari. Composting experiments of pulp and paper mill sludge mixed with monosodium glutamate wastewater by inoculating strain AO- Ⅲ showed that AO-Ⅲ could shorten the time of reaching the high temperature stage（over 80 ℃）from three days to one day, and the cycle of the aerobic thermophilic fermentation was from 20 days to 10 days. The moisture content in the raw mixture was degraded form 55% to 40%. The strain could maintain long-term high temperature and shorten the composting fermentation cycle.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第1期368-371,共4页
Journal of Agro-Environment Science
基金
广州市科技攻关重大项目(2004Z1-E0071)
关键词
造纸污泥
堆肥
高温放线菌
甘蔗兰希氏菌
鉴定
pulp and paper mill sludge
composting
thermophilic actinomycetes
thermoactinomyces sacchari
identification
