摘要
目的:探讨急性氟中毒对小鼠神经系统损害的机制。方法:选用昆明种小鼠24只,分为对照组、低氟组、中氟组、高氟组,每组6只,染氟组分别腹腔注射氟化钠(NaF)8.75、17.53、5 mg/kg,对照组同期注射等剂量生理盐水。分别采用黄递酶(NADPH-d)染色和吖啶橙荧光染色法,观察动物大脑皮层一氧化氮合酶(NOS)活性的变化和皮层DNA及RNA荧光染色强度。结果:与对照组相比,中、低氟组阳性神经元数量明显增多(P<0.05),而高氟组差异不明显(P>0.05);给药组皮层DNA和RNA吖啶橙染色后的荧光强度弱于对照组,且随给药浓度加大强度越弱。结论:急性氟中毒可增强小鼠皮层NOS阳性神经元的表达,对皮层神经元DNA和RNA亦有损伤作用。
Object：The research on the effects of acute fluorine exposed mouse. Methods： In choosing 24 Jimpy mice divided into four group： the control group, the low fluorine group, the suffer fluorine group, the high fluorine group, inject the fluorine exposed group of NaF 8.75、17.5、35 mg/kg,the control group injects the same amount of physiological saline in the same period. The NOS activity in mouse cerebral cortex by NADPH - diaphorasel （NADPH - d） histochemistry was investigated by using NADPH - diap horased （NADPH - d）histochemist ry and Acridine orange fluorescence. Results： In contrast to the control group, the NOS activity of the low fluorine and the suffer fluorine group, are higher than that of the control group（P〈 0.05）, but the high fluorine group are lower than that of the control group（P 〉 0.05）. the fluorescent intensity of DNA and RNA lower. Conclusions： Acute fluorine exposure can lead to boost the NOS activity in mouse cerebral cortex and cortical DNA and RNA can be scathe.
出处
《微量元素与健康研究》
CAS
2008年第1期4-5,9,共3页
Studies of Trace Elements and Health
