摘要
将人神经生长因子基因（hNGF）克隆到pMalC2质粒中，构建了表达产物为麦芽糖结合蛋白（MBP）与hNGF的融合蛋白重组质粒。经由内切酶再水解及DNA序列测定等分析表明，重组质粒含hNGF基因（360bP），该质粒在E·Coli中的表达产物为融合蛋白MBP－hNGF（55kd），光密度扫描表明，其表达量约为30％。
The DNA fragment coding for human nerve growth factor is cloned into pMalc2 vector. The recombinant plasmid constructed can express a product which is the fusion protein of maltose binding protein (MBP) and human nerve growth factor (hNGF). It is showed from the results of endonuclease redigestion and DNA sequence analysis that the recombinant plasmid exactly contains hNGF DNA fragment (360bp).The expression product of the plasmid in E. colt is a fusion protein with the molecular weight of 55kd on SDS-PAGE. Compared with the total protein of cells, the yield of the fusion protein is about 30% by gel optical density scanning.
出处
《高技术通讯》
EI
CAS
CSCD
1997年第5期44-46,共3页
Chinese High Technology Letters
