摘要
采用重氮化法,将磺胺间甲嘧啶(SM1)与牛血清蛋白(BSA)偶联制备合成抗原BSA-SM1,并用作免疫抗原,用紫外扫描法和聚丙烯酰胺电泳法对其进行鉴定,并测得偶联的结合比。结果表明,通过紫外扫描和聚丙烯酰胺电泳法的测定证明偶联成功,偶联的结合比为9∶1。用BSA-SDM免疫BALB/C小鼠,间接ELISA测定多抗上清(pAb)效价,获得了高效价的pAb,为SM1残留免疫学检测方法的建立奠定了基础。
In this paper, Bovine serum albumin （BSA） was used as a protein carrier to couple with semiantigen Sulfamerazine （SM1） by diazotization method. The complete antigen BSA-SM was thus prepared as immunoantigen in enzyme-linked immunosorbent assay （ELISA） protocol. UV spectrophoto metry and Sodium dodecyl-sulfatepolycrylamide gel electrophonesis （SDS-PAGE） were applied to determine it. And the conjugated ratio was estimated. The results indicated that the conjugation was succeed and the conjugated ratio of SM1 and BSA was 9：1. The high-titer anti-SM1 pAb was been produced in serum of immunized BALB/C mice, which made it possible to establish method of SM1 residues in feed and animal food.
出处
《湖北农业科学》
北大核心
2008年第1期87-90,共4页
Hubei Agricultural Sciences
基金
河南科技学院重点科研项目(040111)
关键词
磺胺间甲嘧啶
重氮化法
人工抗原
合成
sulfamerazine
method of diazotization
artificial antigen
synthesis
identification
