摘要
目的建立同时测定止痒消炎水中薄荷脑、冰片和麝香草酚含量的气相色谱测定方法。方法采用气相色谱内标法,FID检测器,HP-INNDW AX石英毛细管柱(30m×250μm,0.25μm),应用程序升温(140℃保持4.1min以后20℃.min-1升至240℃),进样口温度200℃,检测器温度250℃;分流进样,分流比10:1。结果薄荷脑在0.32～3.2mg.mL-1,冰片在0.56～5.6mg.mL-1,麝香草酚在0.16～1.6mg.mL-1内呈良好的线性关系,加样回收率,薄荷脑为99.8%,(RSD=1.3%);冰片为99.2%(RSD=1.5%);麝香草酚为99.8%(RSD=0.9%)。结论该方法简单可靠,适合于止痒消炎水中薄荷脑、冰片和麝香草酚的含量测定。
OBJECTIVE To establish a GC method for simultaneous determination of three constituents in Zhiyang Xiaoyanshui containing Menthol,Bomeol and Thymol. METHODS The internal standard method was employed; Flame ionization detector and capillary column HP-INNDWAX （30 m ×250 μm ×0.25 μm） were used. The column temperature was program-raised . The injector temperature was 200℃ ,and the detector temperature was 250 ℃ ;split injection was employed with 10：1 split ratio. RESULTS Menthol,Bomeol and Thymol presented a good linear correlation within a range of 0.32 ～ 3.2 mg · mL^-1 （ Menthol）, 0.56 ～5.6 mg · mL^-1 （Bomeol） and 0. 16 ～1.6 mg ·mL^-1 （Thymol） , respectively. The average recoveries were 99.8% for Menthol （ RSD was 1.3% ） ,99.2% for Bomeol （ RSD was 1.5% ） and Thymol 99.8% （ RSD was 0.9% ） . CONCLUSION This method is simple, reliable for the quality control of Zhiyang Xiaoyanshui.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2007年第6期505-507,共3页
Chinese Journal of Modern Applied Pharmacy
