摘要
用秋水仙素和2%二甲基亚砜共同处理新疆雪莲种子、胚根和茎段,以诱导多倍体雪莲。结果表明,茎段经过100 mg.L-1秋水仙素处理3 d诱导效果最佳,同时,离体培养其嵌合体苗茎段及叶片,诱导不定芽生成,获得了四倍体细胞比例达到90%以上的植株。
The stem,seed,radicel of Saussurea involucrata Kar. et Kir. were induced with colchicine and 2%DMSO. The results showed that the better effective induction team is that the stem was induced with 100 mg · L^-1 colchicine for 3 d. Furthermore, the stem and leaf of the mixoploid of the 100 mg · L^-1 colchicine were cultivated for 3 d to stimulate the adventitious buds to regenerate and the plants with more than 90% of tetrapolid cell were gained.
出处
《西北农业学报》
CSCD
北大核心
2008年第1期216-220,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
中国科学院西部行动高新技术项目:新疆雪莲规模化组培快繁技术研究(KGCX2-SW-506)
关键词
秋水仙素
新疆雪莲
多倍体
Colchicines
Saussurea involucrata Kar et Kir
Polyploid
