摘要
目的制备单抗CD33导向的三氧化二砷免疫蛋白毫微球(CD33-As2O3-BSA-NP)并检测其特异性结合APL原代细胞的活性。方法通过N-羟基琥珀酰亚胺基-3-(2-吡基二硫)-丙酸酯(SPDP)交联的方法制备免疫蛋白毫微球。玻片凝集实验、免疫荧光法、光镜和电镜、CD33-As2O3-BSA-NP结合的CD33数量测定、检测As2O3免疫毫微球的共价连接与活性。结果CD33-As2O3-BSA-NP的玻片凝集反应,免疫荧光染色均为阳性;光镜下可看见细胞周围结合微球,电镜下可看见细胞伸出伪足紧密地与免疫毫微球结合在一起;每1 g CD33-As2O3-BSA-NP表面偶联的CD33抗体数量是14.5 mg。结论制备的CD33-As2O3-BSA-NP由共价键连接且特异性的结合APL原代细胞。
OBJECTIVE To prepare arsenic trioxide-loaded albumin immuno-nanoparticles （As2O3-BSA-NP） targeted with Monoclonal Antibody CD33 and test its specific killing effect on APL original cells. METHODS Immuno-nanoparticles were prepared by methods of SPDP crosslinking. Immuno-nanoparticles and its activity were tested by slide agglutination test, immunofluorescent assay, microscopy and scanning electron microscopy, the number of CD33 junctioned the surface of As2O3-BSA-NP. RESULTS CD33-As2O3-BSA-NP was positive in slide agglutination test and immunofluorescent assay. Under microscopy, the APL original cells were rounded with immuno-nanoparticles. The scanning electronmicroscopy revealed the albumin immuno-nanoparticles were tightly connected with the APL original cells. The amount of CD33 juncted with the surface of one gram CD33-As2O3-BSA-NP were 14. 5 mg. CONCLUSION CD33-As2O3-BSA-NP might specifically bind against APL original cells by covalent bond.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2008年第2期115-118,共4页
Chinese Pharmaceutical Journal
基金
江西省科技厅重大专项(E031101)
关键词
三氧化二砷
免疫毫微球
制备
Arsenic trioxide
immuno-nanoparticles
preparation
