摘要
以麻疯树油为唯一碳源,从以粉碎的麻疯树种子处理过的土壤中分离筛选出1株脂肪酶活性较高的茵株,初步鉴定为假单胞菌属(Pseudomonas)。实验观察了碳源、氮源、无机盐及发酵工艺对产酶的影响,摇瓶发酵结果表明,该菌株最适产酶培养基的组成是(%,w/v):橄榄油2,酵母膏0.5,(NH_4)_2SO_40.5,MgCl_2·6H_2O 0.5,最适产酶温度为30℃,最佳产酶pH为6.5,转速180r/min,发酵培养36h酶活达到最高,为14.17U/mL。本研究为以麻疯树油为原料酶法生产生物柴油奠定了一定的基础。
A wild lipase-producing strain was isolated from the soil pretreated by Jatropha curcas seed powder with Jatropha oil as sole carbon source and identified as Pseudomonas sp. The fermentation conditions of the lipase-production strain were optimized and the effects of carbon sources, nitrogen sources, and fermentation techniques on the lipase production were studied. The results showed that the optimal medium composition for lipase production was as follows （%, w/v）： olive oil 2.0, yeast extract 0.5, （NH4）2SO4 0.5,MgCl2· 6H2O 0.5. The optimal initial pH of fermentation medium was 6.5. The maximum yield was 14.17 U/mL and obtained when Pseudomonas sp, was shake-cultivated at 30℃ for 36 h with rotation speed of 180r/min.
出处
《现代生物医学进展》
CAS
2008年第2期259-262,共4页
Progress in Modern Biomedicine
基金
国家重大基础研究项目(No.2003CB716000)
国家自然科学基金重点项目(No.20336010)资助
关键词
脂肪酶
假单胞菌
分离
培养
Lipase
Pseudomonas sp.
Screening
Fermentation condition
