摘要
目的检测IL-10和IL-12在结直肠癌组织中含量及术前和术后3周血清浓度并探讨其意义。方法采用ELISA法检测了46例结直肠癌IL-10和IL-12在癌组织和癌周组织中含量及术前和术后1,3周血清浓度,以24例良性结肠息肉患者资料为对照。结果结直肠癌组织IL-10含量高于癌周组织,而癌组织IL-12低于癌周组织;Ⅲ、Ⅳ期癌组织和癌周组织中IL-10含量高于Ⅰ、Ⅱ期,而Ⅰ、Ⅱ期癌组织和癌周组织中IL-12含量高于Ⅲ、Ⅳ。术前和术后1周血清IL-10升高而IL-12降低,术后3周血清IL-10和IL-12浓度接近对照组。瘤内以及血清IL-10和IL-12呈明显负相关。结论结直肠癌细胞可分泌IL-10,抑制IL-12的产生,进而营造免疫抑制微环境。动态检测IL-10和IL-12可反映结直肠癌病期、判断疗效和估计预后。
Objective To detect the level of IL-10 and IL-12 in colorectal carcinoma tissue and serum 3 weeks before and after operation,and evaluate its clinical significance. Methods The content of IL-10 and IL-12 in colorectal carcinoma tissue, surrounding tissue and serum 3 weeks before and after the operation by ELISA assay in 46 cases with colorectal carcinoma. 24 cases of benign colonic polyp were chosen as control. Results The content of IL-10 in carcinoma tissues was higher significantly than that in surrounding tissue,and contra versus about IL-12. The contents of IL-10 were higher in Ⅲand Ⅳstages than that of Ⅰand Ⅱstages ,and contra versus about IL-12. The concentrations of serum IL-10 increased preoperative and postoperative one week, and contra versus about IL-12.3 weeks after operation ,the level of IL-10 and IL-12 reached proximally to control. There was negative correlation between IL-10 and IL-12 in tumor and serum. Conclusions In patients with colorectal carcinoma,carcinoma cell may secrete IL-10 to suppress IL-12,which therefore creates microenvironment of immune suppression. Detecting IL-10 and IL-12 dynamically is helpful to understand the stage of colorectal carcinoma,judge the curative effect and estimate prognosis.
出处
《实用全科医学》
2008年第2期116-117,共2页
Applied Journal Of General Practice
