摘要
乙醇分级丝胶酶解产物得到轻度疏水、低分子量的醇溶丝胶肽和高亲水性、分布在200～4000Da的醇沉丝胶肽。采用DA201-C大孔吸附树脂对乙醇分级的丝胶肽进行脱盐和分离提纯。抗氧化实验结果表明,醇溶丝胶肽具有清除脂溶性自由基DPPH.活性,40%乙醇阶段洗脱得到的组分清除活性提高10%;水溶性的醇沉丝胶肽具有还原力,30%和40%乙醇阶段洗脱得到的组分还原力提高2.5倍。大孔吸附树脂提纯的不同极性的丝胶肽具有抗氧化性,值得深入研究和开发功能产品。
Ethanol soluble sericin peptides （ESSP） containing small hydrophobic peptides and ethanol precipitable sericin peptides （EPSP） consisting of hydrophilic peptides mostly distributed among 200-4000Da were separated from sericin enzymatic hydrolysate by alcohol. Macroporous adsorption resin （ DA201 - C） was used to purify sericin peptides separated by alcohol. The results of antioxidant activity tests showed that ESSP had the oil soluble DPPH free-radical scavenging activity,and the activity of the component eluted with 40% alcohol increased 10% water soluble EPSP had the reducing power,and the reducing power of the two components eluted with 30% and 40% alcohol enhanced 2.5 multiple. Different polar sericin peptides purified using macroporous adsorption resin showed antioxidant activity, so it was valuable to further study and develop their application.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第1期149-152,共4页
Science and Technology of Food Industry
关键词
丝胶肽
抗氧化
大孔吸附树脂
醇溶肽
醇沉肽
sericin peptide
antioxidant
macroporous adsorption resin
ethanol soluble peptide
ethanol precipitable peptide
