摘要
目的:获得最佳的微藻藻胆蛋白提取方法。方法:采用溶胀法、反复冻融法、低浓度氯化钙溶液提取法和玻璃珠处理法等四种方法分别对紫球藻、蔷薇藻和念珠藻三种藻细胞进行破碎,通过测定藻红蛋白的纯度和浓度对四种细胞破碎方法效果进行比较。结果:当细胞密度为1.000g/L时,采用反复冻融法处理紫球藻和蔷薇藻时能够获得最高的藻红蛋白纯度(OD545/OD280),分别为1.250和1.669,藻红蛋白的浓度分别为29.788mg/L和36.026mg/L,细胞密度对藻红蛋白纯度影响较小;低浓度氯化钙溶液提取法能使念珠藻藻红蛋白的纯度(OD545/OD280)达到0.477。结论:紫球藻和蔷薇藻经反复冻融法破碎细胞,藻红蛋白纯度高,提取效果好;而对念珠藻藻红蛋白提纯采用低浓度氯化钙溶液提取法效果好。
Objective： In order to get the best extraction process of B - phycoerythrin from microalgae. Methods： Four kinds of cell disruption processcs （dissolving- bulging, freezing and thawing, low concentration of calcium chloride extraction and beading treated） were used to disrupt three microalge （ Porphyridium cruentum, Rhodella reticulata and Nostoc punctiforme）. The purity and concentration of B - phycoerythrin （ B - PE） were used in the assay.Results： By using freezing and thawing, the highest purity of B - PE for Porphyridium cruenum and Rhodella reticulata were obtained when cell density reached 1.000g/L. The purity and concentration of B - PE for Porphyridium cruentum were 1. 250 and 29. 788mg/L, for Rhadella reticulata were 1.669 and 36.026mg/L, respectively. Nostos punaiforme cells, which was treated with low concentration of calcium chloride extraction, reached the highest purity of B - PE（OD545/OD280 = 0.477） when cell concentration was 1.000g/L. Conclusion： By using freezing and thawing, tire highest purity of B - PE and the higher extract yield for Porphyridium cruenum and Rhodella reticulata were obtained. Low concentration of calcium chloride extraction can be used for obtainning higher purity of B - PE from Nostos punctiforme.
出处
《生物技术》
CAS
CSCD
2008年第1期55-58,共4页
Biotechnology
基金
福建省发展和改革委员会项目资助(闽计投资[2003]203)
关键词
藻红蛋白
提取
细胞破碎
微藻
B - phycoerythrin
extraction
cell disruption
microalgae
