摘要
以17β-雌二醇(E2)诱导雄性唐鱼产生卵黄蛋白原(Vtg),采用SephacrylS-300凝胶过滤层析柱提纯了雄鱼整体匀桨液的Vtg。结果显示,已确定被纯化的唐鱼Vtg在4%—7.5%Native—PAGE电泳中分子量为440kD左右,能同时被考马斯亮蓝、糖蛋白、磷蛋白、脂蛋白染色方法同时染色,是一种富含糖、磷、脂的蛋白。与对照组作比较,17β-雌二醇暴露组其体重明显下降(P<0.01),精巢发育滞后。结果表明,17β-雌二醇能够诱导雄性唐鱼产生大量的Vtg,并影响到其生长及精巢发育。雄性唐鱼Vtg可作为环境雌激素监测的有效生物学标记物。
Vitellogenin （Vtg） was purified from whole body homogenate （WBH） of estradiol-17β （E2） treated male Tanichthys albonubes by gel filtration （Sephacryl S-300）. The results showed that the Vtg appeared to exist as homotrimer of approximately 440kD through native polyacrylamide gel electrophoresis （PAGE）. Vtg was characterized as a phospholipoglycoprotein by native-PAGE and staining of gels for CBB R-250, for carbohydrates with periodic acid-schiff reagent, for phosphorus with methyl green and for lipids with sudan black B. Body weight decreased （P〈0.01） and the development of sperm was delayed after E2 treatment when compared with the control group of male fish. The results indicated that E2 can induce male fish to produce large quantities of Vtg and have a negative effect on its growth and gonad development. It also indicated that Vtg of the male T. albonubes could be as a valid biomarker for environmental estrogens.
基金
广东省科技计划项目(2004B40101015
2006B36601008)
关键词
17Β-雌二醇
卵黄蛋白原
精巢
发育
唐鱼
17β-estradiol
Vitellogenin
Sperm
Development
Tanichthys albonubes
