摘要
利用设计合成的1对北方根结线虫(Meloidogyne hapla)特异性寡聚核苷酸引物Mh-F/Mh-R,以北方根结线虫、南方根结线虫、花生根结线虫和爪哇根结线虫全基因组DNA为对照,对采自云南文山、砚山、马关、蒙自等地区的三七根结线虫全基因组DNA进行PCR特异性扩增。结果表明,设计合成的引物能从北方根结线虫和供试的4个三七病原根结线虫全基因组DNA中扩增到462bp长度的分子片段,而南方根结线虫、花生根结线虫和爪哇根结线虫的全基因组DNA无扩增产物。表明4个地区的三七病原根结线虫种群均为北方根结线虫,该对引物可用于三七根结线虫的分子鉴定。
Based on the genomic rDNA sequence, a pair of oligonucleotide primer which was specific for M. hapla, was designed. DNA of nematodes extracted from Wenshan population, Yanshan population, Magnan population, Mengzi populagtion and other 4 Meloidogyne spp. populations （M. hapla,M, incognita,M, arenaria and M. javanica） were amplified with the primers. The results showed that about 462bp fragments were amplified from genomie DNA of Wenshan population, Yanshan population, Maguan population, Mengzi populagtion and M. hapla, but other Meloidogyne spp. （M. incognita,M, arenaria and M.javanica） populations had no amplification products. It indicated that Wenshan population,Yanshan population,Maguan population and Mengzi populagtion were M. hapla. The pair of primers would be useful for molecular identification and detection of root-knot nematode in P. notoginseng.
出处
《西南农业学报》
CSCD
2008年第1期100-102,共3页
Southwest China Journal of Agricultural Sciences
基金
云南省科技攻关项目(2005NG07)
关键词
三七
根结线虫
PCR(聚合酶链武反应)
鉴定
Panax notoginseng
root-knot nematede
PCR（ Polymerase Chain Reaction）
identification
