摘要
通过单因子试验和正交试验对富硒益生菌的培养条件进行了优化，研究了温度、培养时间、培养基中无机硒浓度、接种量、初始pH对益生菌产量和有机硒转化率的影响。确定工业化生产富硒益生菌的优化培养条件：在初始pH6．2面粉培养基中，添加4种益生菌、总接种量为5％，培养基中无机硒浓度10μg／mL，温度h34℃，培养时间h36h，在该培养条件下，4种益生菌均达到10^8～10^9CFU／mL，有机硒含量达到9。41μg／mL、转化率为93.82％。
Single-factor tests and orthogonal test were done to optimize the cultural conditions on selenium-enriched probiotics to ascertain the conditions for industrial produce. Temperature,culture durations,inorganic-selenium concentration,inoculum sizes,the initial pH were studied. It was concluded that four kinds of probiotic were fermented in flour medium, with 5% inoculum sizes and 10mg/mL inorganic-selenium concentration, which were cultured for 36h at 34℃ with the initial pH of the medium of 6.2. Under the above mentioned conditions, 93.82% inorganic-selenium was converted into organic-selenium and the number of four kinds of probiotic was reached 10^8～10^9CFU/mL.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2007年第12期56-59,共4页
Food and Fermentation Industries
基金
国家自然科学基金项目(N0.30671 547)
广东省教育部产学研科技合作项目(No.2006d90204008)
关键词
硒
益生菌
工业化生产
培养条件
Selenium
probiotics
industrial produce
cultural conditions
