摘要
目的探讨卵巢颗粒细胞中凋亡相关蛋白bcl-2、bax的表达与妊娠结局的关系。方法选择67例适合进行体外受精-胚胎移植(IVF-ET)手术的不孕症患者,通过免疫组织化学方法检测卵泡液中卵巢颗粒细胞凋亡相关蛋白bcl-2和bax的表达率。同时分析其与优质胚胎数和妊娠结局的关系。结果IVF-ET患者优质胚胎≥2个组与无或优质胚胎<2个组其颗粒细胞bc1-2蛋白及bax蛋白阳性率比较,差异有统计学意义。临床妊娠组与非临床妊娠组其颗粒细胞bc1-2蛋白及bax蛋白阳性率比较,差异有统计学意义。结论卵巢颗粒细胞凋亡相关蛋白bcl-2、bax的表达与优质胚胎数有关,研究卵巢颗粒细胞的bcl-2、bax蛋白表达可以预测IVF-ET妊娠结局。
Objective To investigate the expression of apoptosis-related proteins bcl-2 and bax in granulosa cells （GCs） in follicular fluid and its relationship with pregnancy outcome after in vitro fertilization and embryo transfer （IVF-ET）. Methods Sixty-seven women undergoing IVF were analyzed. The expression of bcl-2 and bax proteins in GCs in follicular fluid were detected by immunohistochemistry. Results Expression of bcl-2 and bax proteins had significant difference between patients with less than two superior quality embryos and patients with two or more, between clinical non-pregnant patients and clinical pregnant patients. Conclusion The results indicate that the expression of apoptosis-related proteins bcl-2 and bax have relevance to the embryo quality after IVF. Expression of bcl-2 and bax protein can predict the pregnancy outcome after IVF-ET.
出处
《山西医科大学学报》
CAS
2008年第2期176-179,共4页
Journal of Shanxi Medical University
