摘要
目的探讨模拟心理训练对机体受到外界损害性应激时的影响。方法32只Wister大鼠随机均分为空白对照组(A组)、心理训练组(B组)、单纯应激组(C组)、心理训练应激组(D组)。A组不给予心理训练也不给予应激,B组给予心理训练但不给予应激,C组给予电击、强声、强光多种损害性应激,但不予训练,D组在进行4周的心理训练后,随C组一同施加应激,应激后24h进行旷场实验,然后乙醚麻醉各组大鼠,心脏取血5ml,以酶联免疫法测定血清β-内啡肽(β-EP)、皮质酮、IL-1β的含量。结果经过心理训练的B、D组旷场行为得分明显低于未经训练的A、C组;应激后大鼠血清中的β-EP、皮质酮水平明显升高,而IL-1β水平明显降低;经过心理训练的大鼠(D组)遭受到损害应激时血清中β-EP、皮质酮的水平显著低于C组,而IL-1β的水平显著高于C组。结论心理训练可减轻损害性应激对大鼠内分泌和免疫功能的抑制作用,增加机体对抗应激的储备能力。
Objective To reproduce an animal model of psychological simulation training,and to investigate the influence of psychological training on the ability of counter physical stress.Methods Thirty-two Wister rats were assigned to four groups,8 for each,namely control group（group A）,psychological training group（group B）,stress group（group C）and psychological training prior to stress（group D）.Rats in group A received neither psychological training nor stress,group B received psychological training without stress,group C received stimulation including stress of strong noise and strong light,but without psychological training,and group D,after psychologically training for 4 weeks,received the same stress stimulation as group C.Twenty-four hours after stress,open-field experiment was done on group C and D.At last,rats in all the 4 groups were anesthetized by ether and 5ml of blood was drawn from each rat in order to detect the content of beta-endorphin（β-EP）,corticosterone and IL-1β.Results In the open-field experiments,the psychologically trained groups（B and D）showed remarkably lower scores than the un-trained groups（A and C）.The levels of β-EP and corticosterone were significantly elevated in rats after receiving stress stimulation,while that of IL-1β lowered dramatically.The level of β-EP and corticosterone in psychological training group（group D）was remarkably lower,while that of IL-1β was higher,than the stress group（group C）during the stress.Conclusion Psychological training can lessen the inhibitory effects of harmful stress on endocrine and immunologic functions,and enhance the physical ability to confront stress.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2007年第12期1240-1242,共3页
Medical Journal of Chinese People's Liberation Army
基金
全军医学科研"十五"计划指令性课题(01L005)
关键词
适应
心理学
应激
皮质酮
白细胞介素1
Β-内啡肽
adaptation,psychological,stress,corticosterone,interleukin-1,beta-endorphin
