摘要
目的:观察氯胺酮预处理对大鼠急性胃黏膜病变(stressulcer)后一氧化氮(NO)的影响及胃黏膜溃疡指数(ulcerindex,UI)的变化,探讨其可能的保护机制。方法:取健康雄性Wistar大鼠40只,随机分为正常对照组(N组)、应激性溃疡组(S组)、氯胺酮预处理组(K1、K2、K3组),每组8只。采用水浸束缚应激方法制作急性胃黏膜病变模型,于应激6h后检测血浆、胃黏膜NO水平、UI和大体及光镜下胃黏膜损伤程度及组织学改变。结果:氯胺酮预处理组与S组比较胃黏膜损伤程度明显减轻,并且氯胺酮预处理组大鼠血浆、胃黏膜NO水平明显增加。结论:氯胺酮预处理对大鼠急性胃黏膜病变有保护作用,其机制可能与氯胺酮改变血浆、胃黏膜NO水平等因素有关。
Objective To observe the effect of ketamine pretreatment on stress ulcer in rats and the changes of nitric oxide （NO） and ulcer index （UI）, and explore the possible mechanism of protection. Methods Forty rats were randomly divided into five groups, normal group （group N）, stress ulcer group （group S）, and ketamine groups （group K1, K2, and K3）. The murine model of water immersion restraint stress was constructed. Six hours after stress, plasma and gastric mucosal NO levels were detected, UI was measured, the degree of gastric mucosa injury was observed grossly, and the histological changes were examined microscopically. Results As compared with group S, the degree of gastric mucosa injury was markedly declined in ketamine groups while plasma and gastric mucosal NO levels were obviously increased. Conclusion Ketamine pretreatment has a protective effect on stress ulcer, whose mechanisms may involve the capacity of ketmine to alter the levels of plasma and gastric mucosal NO.
出处
《实用医学杂志》
CAS
2008年第2期179-181,共3页
The Journal of Practical Medicine
关键词
消化性溃疡出血
氯胺酮
胃黏膜
一氧化氮
Peptic ulcer hemorrhage Ketamine Gastric mucosa Nitric oxide
