摘要
从污水样品筛选出能利用甲醇的菌株A和B，经16S rDNA测序分析，鉴定为芽孢杆菌（Bacillus sp．KM01）和肺炎克氏杆菌（Kleb-siella pneumoniae）。甲醛耐受能力的测试表明，这两种分离菌对甲醛具有较强的耐受能力，能在含8～15mmol／L甲醛的LB培养基上生长。Southern杂交分析结果说明，这两株菌的基因组中有兼性甲基营养菌每磷酸己酮糖合成酶（HPS）和6-磷酸果糖异构酶（PHI）基因的同源序列。以pUC118为载体构建了这两种分离菌的基因组文库，进一步的检测结果说明所构建的基因组文库质量符合要求。
The strain A and B with CH3OH-utilization ability were isolated from sewage samples. By sequence analyses for 16S rDNA,the two isolated swains were identified as Bacillus sp. KM01 and Klehsiella pneumoniae, respectively. The test with HCHO showed they were well tolerant to HCHO and could grow on LB medium with 8 - 15 mmol/L HCHO. Southern hybridization analyses revealed that the homologs of 3-Hexulose-6-phosphate synthase （HPS） and 6-phospho-3-hexuloisomerase （PHI） genes from a facuhative methylotrophic bacterium, Mycobacterium gastri MB19, were presented in the genomes of the isolated swains, pUC118 being used as the vector, genomic DNA libraries were constructed for the isolated swains. The further analyses showed that the quality of the libraries was satisfied for the requirements for gene screening.
出处
《安徽农业科学》
CAS
北大核心
2008年第3期978-981,1009,共5页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(30670163)
教育部留学回国人员科研启动基金项目(2005-55)
云南省中青年学术与技术带头人培养费(2004PY01-5)
昆明理工大学人才培养费联合资助
