摘要
目的探讨梅毒血清中非特异性梅毒螺旋体抗体对艾滋病病毒(HIV)明胶颗粒凝集试验的影响。方法采用回顾性调查的方法,用快速血浆反应素环状卡片试验(RPR)、HIV明胶颗粒凝集试验(HIV1/2-PA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)以及Abbot HIV胶体硒快速试验(Determine HIV-1/2),同时检测性病门诊保存的96份梅毒血清及96份健康对照者血清。结果96份梅毒血清中有32份HIV1/2-PA结果被干扰而无法判读(致敏颗粒和非致敏颗粒同时凝集),其他64份血清HIV1/2-PA结果为阴性。将96份梅毒血清按滴度分成11组,观察到随着RPR滴度的升高,HIV1/2-PA结果出现干扰现象的频率也升高。RPR滴度>1∶8的标本出现此现象的比例与≤1∶8的标本相比,两者差异有统计学意义(χ2=74.73,P<0.005)。对9例出现HIV1/2-PA结果干扰的梅毒患者进行随访,在苄星青霉素规范治疗后6个月,用HIV1/2-PA测试其血清,原结果中的干扰现象消失。比较这9例患者治疗前后RPR和TPPA滴度的变化发现,RPR的滴度均下降至≤1∶8,而TPPA的滴度则有4例升高,有4例未变化。5份RPR阳性、滴度均<1∶8。TPPA阴性标本,在HIV1/2-PA试验中未出现结果被干扰现象。结论高滴度(>1∶8)的非特异性梅毒螺旋体抗体,是HIV1/2-PA出现致敏颗粒和非致敏颗粒同时凝集这一干扰现象的主要原因。
Objective To investigate the effect of nontreponemal antibodies on HIV gelation particle agglutination test in syphilis cases. Methods The nontreponemal antibodies, treponemal antibody and HIV antibody in serum samples from 96 syphilis patients and 96 healthy controls were tested with rapid plasma reagent circle card test（RPR）, treponema pallidum particle assay（TPPA）, HIV gelatin particle agglutination test（HIV1/2- PA） and Determine HIV-1/2 test. Results In 96 syphilis serum samples, 32 samples showed agglutination pictures in both sensitive and unsensitive particles HIV1/ 2 - PA test and so the results were undeterminable. These 96 syphilis serum samples were divided into 11 groups according to nontreponemal antibodies titers（RPR titers）, which showed that interfering results of HIV1/2 - PA occurred more frequently with RPR titer rising. The undeterminable results occurred with a significant difference between the group with RPR titers up 1：8（ 〉 1：8）and the group with RPR titem down 1：8 （≤1：8） （χ^2= 74.73 P 〈 0.005）. Nine cases with undeterminable HIV1/2- PA results were followed up. They were tested with HIV1/2- PA in 6 months after the treatment with benzathine penicillin. The follow up results showed that the phenomenon of agglutinating unsensitive and sensi- tive particles disappeared and their HIV antibodies all were negative. On the other hand, their RPR titers all dropped down to 1 ： 8 （≤1 ： 8） and there were 4 cases where the treponemal antibody titers rose and 4 cases where the antibody remained unchanged. The phenomenon of agglutinating unsensitive and sensitive particles did not appear in 5 samples, which were positive in RPR and negative in TPPA, but their RPR titers were all under 1 ： 8. Conclusions High titers of nontrepone- mal antibodies（ 〉 1：8）in serum samples were essential to interfering HIV1/2 - PA results.
出处
《中国艾滋病性病》
CAS
2008年第1期25-27,共3页
Chinese Journal of Aids & STD
