摘要
目的为了解钒酸盐氧化法测定血清胆红素的可行性,进行方法学评价。方法钒酸盐氧化法与重氮法测定胆红素的方法进行了比较,并对钒酸盐氧化法的重复性试验、线性试验、干扰试验、回收试验进行了评价。结果总胆红素测定批内CV%为0.8%,批间CV%为1.3%,平均回收率为101.1%,线性达581.2μmol/L,与重氮法比较,Y(本法)=0.935X(重氮法)+1.335,t=0.02,P>0.5,r=0.998,血红蛋白浓度和甘油三酯分别高达3.4g/L和7.3mmol/L不干扰。结论结果表明钒酸盐氧化法测定血清胆红素具有简单、快速、稳定、灵敏、准确、重复性好。溶血、乳糜对测定表现为较微的干扰。可作为常规法使用。
Objective We introduce Oxidation method of measuring bilirubin in serum by Vanadic acid. Methods We use the vanadate oxidation reagent of bilirubin to determinate serum total bilirubin simultaneously, take a comparison with the method of improved Diazo methods and also take run -in -run test,linear test, interfere test, recovery, test of vanadate oxidation. Results The CVs of determination total bilirubin were 0.8% for intra - run and 1.3% for mid -run. The average recovery rate wasl01.1% for total bilirubin. Linear range of total bilirubin can attach to 581.2μmol/L. The result of the Vanadate oxidation（Y） has a good correlation with Diazo method. Their＇ s regression equation and r, t,p value were, TBIL Y （Vanadate） = 0. 935X（Diazo） ＋ 1. 335, t = 0.02, P 〉 0.5, r = 0.998. Hemoglobin and chylomicronemia have rarely interfere. Conclusion The method is single, rapid, stable, sensitive and accurate. It has the ability to anti - interference of hemoglobin and chylomicronemia. It can be used in the routine determination of bilirubin.
出处
《哈尔滨医药》
2008年第1期5-6,共2页
Harbin Medical Journal
关键词
胆红素
钒酸盐氧化法
重氮法
Bilirubin
Vanadate oxidation method
Diazo method
