摘要
研究了自然的和热凝固的良性前列腺增生(BPH)组织在590～1064nm光谱范围的光学特性及其差异,实验采用带积分球附件的分光光度计以及反向倍增法获取组织样品的吸收和散射特性参数。结果表明:热凝固导致BPH组织在590～1064nm光谱范围的吸收系数明显地减小的,自然的和热凝固的BPH组织的吸收系数都有一个峰值在990nm处,其值分别为0.438和0.416mm-1,自然的和热凝固的BPH组织的吸收系数的最大差异在1064nm,其值为86.79%,其最小差异在920nm,其值为4.74%。热凝固导致BPH组织在600～1064nm光谱范围的约化散射系数明显地增大,而在590nm处,热凝固导致BPH组织的约化散射系数却是明显地减小,自然的和热凝固的BPH组织的约化散射系数都有一个峰值在970nm处,其值分别为1.090和1.449mm-1,其另一个峰值都在1050nm处,其值分别为1.116和1.627mm-1,自然的和热凝固的BPH组织的约化散射系数的最大差异在1060nm,其值为47.73%,其最小差异在600nm,其值为4.86%。
The optical properties and their differences of native and coagulated human benign prostatic hyperplasia （BPH） tissues were studied in the spectral range from 590 to 1 064 nm in vitro. The measurements were performed using a spectrophotometer with an integrating sphere attachment, and the absorption and scattering properties were assessed from these measurements using the inverse adding-doubling method. The results of measurement showed that the thermal coagulation of BPH tissues induced obviously the decrease in the absorption coefficients in the spectral range from 590 to 1 064 nm. The peaks in the absorption coefficients for native and coagulated BPH tissues were respectively 0. 438 and 0. 416 mm 1 corresponding to the same wavelength of 990 nm, the maximum difference in the absorption coefficients of native and coagulated BPH tissues is 86.79% at 1 064 nm, and the minimum difference is 4.74% at 920 nm. The thermal coagulation of BPH tissues induced an increase in the reduced scattering coefficients in the spectral range from 600 to 1 064 nm obviously, and induced a decrease in the reduced scattering coefficients at 590 nm obviously. The peaks in the reduced scattering coefficients for native and coagulated BPH tissues were respectively 1. 090 and 1. 449 mm^-1 corresponding to the same wavelength of 970 nm, and other peaks in the reduced scattering coefficients for native and coagulated BPH tissues were respectively 1. 116 and 1. 627 mm^-1 corresponding to the same wavelength of 1 050 nm, the maximum difference in the reduced scattering coefficients of native and coagulated BPH tissues is 47.73% at 1 064 nm, and the minimum difference is 4.86% at 600 nm.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2008年第2期394-398,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(60378043
30470494)
广东省自然科学基金项目(015012,04010394)
广东省科技计划项目(2004B10401011)资助
关键词
热凝固
人良性前列腺增生组织
吸收和散射特性
积分球
Thermal coagulation
Human benign prostatic hyperplasia tissues
Absorption and scattering properties
Integrating- sphere
